Aberrant KIF23 expression is associated with adverse clinical outcome and promotes cellular malignant behavior through the Wnt/β-catenin signaling pathway in Colorectal Cancer
Zhiyu Ji, Aoning Mi, Mengmeng Li, Quanying Li, Changjiang Qin
Abstract
The aim of the present study was to reveal the clinicopathological significance and prognostic role of kinesin family member 23 (KIF23) in colorectal cancer (CRC) and characterize its biological function and the underlying mechanisms. Methods: Bioinformatics analysis, immunohistochemistry, Western blot and qRT-PCR were utilized to investigate the expression of KIF23 in CRC tissues. The CCK-8 assay, wound healing assay and Matrigel assay were used to detect cell proliferation, migration and invasion in vitro. Western blot, immunofluorescence staining and cell function experiment were performed to explore the underlying mechanism.
Topics & Concepts
Wnt signaling pathwayCateninColorectal cancerCancer researchAdverse Outcome PathwayBeta-cateninMedicineCancerSignal transductionOncologyInternal medicineBiologyComputational biologyGeneticsMicrotubule and mitosis dynamicsWnt/β-catenin signaling in development and cancerHedgehog Signaling Pathway Studies