A monomeric StayGold fluorescent protein
Esther Ivorra-Molla, Dipayan Akhuli, Martin B. L. McAndrew, William Scott, Lokesh Kumar, Saravanan Palani, Masanori Mishima, Allister Crow, Mohan K. Balasubramanian
Abstract
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.
Topics & Concepts
FluorescencePhotobleachingMonomerGreen fluorescent proteinChemistryDimerDerivative (finance)Fluorescent proteinYellow fluorescent proteinBiophysicsFluorescence recovery after photobleachingBrightnessBiochemistryBiologyPolymerOrganic chemistryOpticsEconomicsGenePhysicsFinancial economicsAdvanced Fluorescence Microscopy TechniquesCancer Research and TreatmentsPhotosynthetic Processes and Mechanisms