Integrated transcriptomics, proteomics and metabolomics to identify biomarkers of astragaloside IV against cerebral ischemic injury in rats
Xiaoyu Wei, Yu He, Haitong Wan, Junjun Yin, Bingying Lin, Zhishan Ding, Jiehong Yang, Huifen Zhou
Abstract
. We further validated these targets using 4D-PRM proteomics and found that seven targets were significantly different, including Aprt, Atic, Gaa, Galk1, Glb1, Me2, and Hexa. We aimed to uncover the mechanism of AS-IV in the treatment of ischemic brain injury through a comprehensive strategy combining transcriptomics, proteomics, and metabolomics.
Topics & Concepts
MetabolomicsProteomicsIschemic injuryAstragalosideTranscriptomeMedicinePharmacologyBioinformaticsBiologyChemistryInternal medicineGene expressionIschemiaBiochemistryChromatographyGeneHigh-performance liquid chromatographySphingolipid Metabolism and SignalingNeurological Disease Mechanisms and TreatmentsNeuroinflammation and Neurodegeneration Mechanisms