Comparison of the Cytotoxic Effect of 3D-Printed Resins, Resin-based CAD/CAM blocks and Composite Resin
Nevin AYDIN, S. Karaoğlanoğlu, Aysun Kılıç-Süloğlu, Sencar Öztürk
Abstract
Background: This study investigated the cytotoxic effects of 3D-printed permanent resins, resin-based CAD/CAM blocks and composite resin on human gingival fibroblast (HGF-1) and mouse fibroblast (L929) cell line. Material and Methods: 3D-printed permanent resins (Crowntec and Permanent Crown), resin-based CAD/CAM blocks (Vita Enamic and Brilliant Crios) and composite resin (Clearfill Majesty Posterior) were used in the study. Samples were prepared from the planned materials and kept in DMEM according to ISO 10993-12:2021 standard (3 cm2/ml). The cytotoxic effect of the materials on HGF-1 and L929 cells was examined by MTT test at the end of 24 and 72 h. Two-way analysis of variance test (ANOVA) was used to analyze cell viability data. Results: >0.05). Conclusions: 3D-printed resin, CAD/CAM block, Composite resin, Cytotoxicity, Human Gingival Fibroblast.