A simple and practical solvent system selection strategy for high-speed countercurrent chromatography based on the HPLC polarity parameter model
Xiaohan Zhu, Pengcheng Li, Jintian Tang, Yanqi Su, Mi Xiao, Hongkun Xue, Xu Cai
Abstract
Hance were selected, and the two main components (mangostin and quercetin) were isolated from their extracts, respectively. The separation results showed that the predicted compound polarities were sufficient to meet the HSCCC separation requirements. Meanwhile, this method required only 1 to 2 HPLC analyses with reference compounds, greatly improved the efficiency of the HSCCC solvent system selection, and shortened the experimental time. The polarity parameter model was a fast and efficient analysis method for the selection of an appropriate HSCCC solvent system.
Topics & Concepts
Countercurrent chromatographyHigh-performance liquid chromatographyChromatographyChemistryPolarity (international relations)SolventSolvent polarityOrganic chemistryCellBiochemistryChromatography in Natural ProductsGinseng Biological Effects and ApplicationsNatural product bioactivities and synthesis