Sub-Acute Treatment of Curcumin Derivative J147 Ameliorates Depression-Like Behavior Through 5-HT1A-Mediated cAMP Signaling
Jianxin Li, Ling Chen, Gaowen Li, Xiaojuan Chen, Sisi Hu, Liang Zheng, Victor Luria, Jinpeng Lv, Yindi Sun, Ying Xu, Yingcong Yu
Abstract
BACKGROUND: receptors and downstream cAMP-BDNF signaling. METHODS: agonist or its antagonist was used to determine which 5-HT receptor subtype is involved in the antidepressant-like effects of J147. The downstream signaling molecules such as cAMP, PKA, pCREB, and BDNF were also measured to determine the mechanism of action. RESULTS: receptor antagonist NAS-181 did not appreciably alter the effects of J147 on depression-like behaviors. Moreover, pretreatment with NAD-299 blocked J147-induced increases in cAMP, PKA, pCREB, and BDNF expression in the hippocampus, while 8-OH-DPAT enhanced the effects of J147 on these proteins' expression. CONCLUSION: -dependent cAMP/PKA/pCREB/BDNF signaling.