Syncytium cell growth increases Kir2.1 contribution in human iPSC-cardiomyocytes
Weizhen Li, Julie Han, Emilia Entcheva
Abstract
We identify cell culture density and cell-cell contact as an important factor in determining the expression of a key ion channel at the transcriptional and the protein levels, KCNJ2/Kir2.1, and its contribution to the electrophysiology of human induced pluripotent stem cell-derived cardiomyocytes. Our results indicate that studies on isolated cells, out of tissue context, may underestimate the cellular ion channel properties being characterized.
Topics & Concepts
Cell biologySyncytiumElectrophysiologyInduced pluripotent stem cellPotassium channelBiologyMembrane potentialIon channelInward-rectifier potassium ion channelCell cultureCardiac electrophysiologyChemistryBiophysicsBiochemistryNeuroscienceEmbryonic stem cellGeneticsReceptorGeneNeuroscience and Neural EngineeringCardiac electrophysiology and arrhythmiasIon channel regulation and function