An endoplasmic reticulum-targeting fluorescent probe for imaging ˙OH in living cells
Yanyan Zhao, Hongyu Li, Ziyin Chai, Wen Shi, Xiaohua Li, Huimin Ma
Abstract
The hydroxyl radical (˙OH) in the endoplasmic reticulum has not been studied thoroughly due to the lack of proper methods. Here, an endoplasmic reticulum-targeting fluorescent probe for detecting ˙OH is reported. With this probe, the action of ˙OH in the endoplasmic reticulum has been imaged in living cells.
Topics & Concepts
Endoplasmic reticulumFluorescenceChemistryBiophysicsLive cell imagingUnfolded protein responseFluorescence-lifetime imaging microscopyCell biologyFluorescent proteinGreen fluorescent proteinBiochemistryBiologyCellPhysicsQuantum mechanicsGeneEndoplasmic Reticulum Stress and DiseaseAutophagy in Disease and TherapyLipid Membrane Structure and Behavior