Progressive stretch enhances growth and maturation of 3D stem-cell-derived myocardium
Kun Lü, Thomas Seidel, Xiaochun Cao-Ehlker, Tatjana Dorn, Aarif M.N. Batcha, Christine M. Schneider, Marie Semmler, Tilmann Volk, Alessandra Moretti, Andreas Dendorfer, Roland Tomasi
Abstract
With the intention to simulate postnatal heart development, we have established techniques of tissue assembly and biomimetic culture that avoid tissue shrinkage and yield muscle fibers with contractility and compliance approaching the properties of adult myocardium. This study demonstrates that cultivation under progressive stretch is a feasible way to induce growth and maturation of stem cell-derived myocardium. The novel tissue-engineering approach fulfills important requirements of disease modelling and therapeutic tissue replacement.
Topics & Concepts
Stem cellCell biologyChemistryBiologyTissue Engineering and Regenerative MedicineElectrospun Nanofibers in Biomedical ApplicationsAdditive Manufacturing and 3D Printing Technologies