Amyloid precursor protein and presenilin‐1 knock‐in immunodeficient mice exhibit intraneuronal Aβ pathology, microgliosis, and extensive neuronal loss
Pravin Yeapuri, Jatin Machhi, Emma G. Foster, Rana Kadry, Shaurav Bhattarai, Yaman Lu, Susmita Sil, Roshan Sapkota, Shefali Srivastava, Mohit Kumar, Tsuneya Ikezu, Larisa Y. Poluektova, Howard E. Gendelman, R. Lee Mosley
Abstract
Abstract INTRODUCTION Transgenic mice overexpressing familial Alzheimer's disease (AD) mutations (FAD) show non‐physiological traits, and their immunocompetent backgrounds limit their use in AD immunotherapy research. Preclinical models that reflect human immune responses in AD are needed. METHODS Using CRISPR‐Cas9, we developed single (NA) and double (NAPS) knock‐in (KI) amyloid precursor protein (APP) KM670,671NL (Swedish) and presenilin 1 (PS 1) M146V FAD mutations on an immunodeficient NOG (NOD.Cg‐Prkdc scid Il2rg tm1Sug /JicTac) background. The models were confirmed by Sanger sequencing and evaluated for AD‐like pathology. RESULTS Both NA and NAPS mice developed pathology without overexpression artifacts. Mutation‐induced upregulation of APP‐CTF‐β led to intraneuronal human amyloid beta (Aβ) (6E10) deposits and amyloid‐associated microgliosis as early as 3 months, which increased with age. The addition of the PS 1 M146V mutation doubled the amyloid load. The models displayed broad neuronal loss, resulting in brain atrophy in older mice. DISCUSSION These models replicate intraneuronal amyloid pathology and, with human immune reconstitution potential, enable novel studies of human immune responses in AD. Highlights A novel Alzheimer's disease (AD) knock‐in (KI) mouse was developed and characterized on an immunodeficient NOG background. The model provides a platform for human immune studies and the evaluation of immunotherapies for AD. The KI mice demonstrate intraneuronal Aβ deposits and amyloid‐associated microglial reactions. KI mice demonstrate extensive neuronal loss. Human immune reconstitution enables studies of infectious AD co‐morbidities, such as the human immunodeficiency and herpes simplex viruses.