Plasma membrane phospholipid signature recruits the plant exocyst complex via the EXO70A1 subunit
Lukáš Synek, Roman Pleskot, Juraj Sekereš, Natalia Serrano, Nemanja Vukašinović, Jitka Ortmannová, Martina Klejchová, Přemysl Pejchar, Klára Batystová, Małgorzata Gutkowska, Edita Janková Drdová, Vedrana Marković, Tamara Pečenková, Jiří Šantrůček, Viktor Žárský, Martin Potocký
Abstract
Significance Targeted secretion of the plasma membrane (PM) and cell wall material drives plant cell polarity and morphogenesis. Vesicle-tethering complex exocyst regulates this vital process. We uncovered that one of the exocyst subunits, EXO70A1, is responsible for recruiting the plant exocyst to the PM. EXO70A1 binds PM via interactions with several phospholipids that specifically contribute to the PM–lipid signature in plant cells. We demonstrated that in plants, phosphatidylinositol 4-phosphate and phosphatidic acid orchestrate the exocyst recruitment to the PM, and their action is mutually substitutable. Moreover, our results suggest that the PM net negative charge is a principal driving force at the membrane–protein interface. Our study provides a blueprint for future studies analyzing protein–membrane interactions in plant cells.