Extracellular vesicles enriched with an endothelial cell pro-survival microRNA affects skin tissue regeneration
Hugo Fernandes, Alessandra Zonnari, Ricardo C. de Abreu, Sezin Aday, Marta Barão, Inês Albino, Miguel Lino, Ana F. Branco, Cátia Seabra, Tânia Barata, Ermelindo C. Leal, José Guilherme Tralhão, Lino Gonçalves, Alwin de Jong, Hendrika A. B. Peters, Margreet R. de Vries, Paula A. da Costa Martins, Paul H.A. Quax, Lino Ferreira
Abstract
, that miR-425-5p-modulated sEVs were (1) capable of enhancing the survival of ECs exposed to ischemia-mimic conditions, and (2) efficiently internalized by skin cells. Finally, using a streptozotocin-induced diabetic wound healing mouse model, we showed that, compared with miR-scrambled-modulated sEVs, topical administration of miR-425-5p-modulated sEVs significantly enhanced wound healing, a process mediated by enhanced vascularization and skin re-epithelialization.