RP-HPLC method for simultaneous determination of escitalopram oxalate and flupentixol HCl in tablet dosage form
Wrushali A. Panchale, Shivrani W. Nimbokar, Bhushan R. Gudalwar, Ravindra L. Bakal, Jagdish V. Manwar
Abstract
RP-HPLC method was developed for simultaneous determination of escitalopram oxalate (ESC) and flupentixol HCl (FLU) in tablet dosage form. Mobile phase consisting of mixture of acetonitrile and potassium phosphate buffer (pH 7.0 with 0.1% triethylamine) in the ratio 60: 40 at flow rate of 1ml/min using C18 Grace (250mmX 4.6mm) column at 231 nm. The retention time of ESC with FLU was found to be 2.96 min and 6.98 min, respectively. The linearity range for ESC with FLU observed was 5-25 µg/ml and 10-50 µg/ml, respectively. Method was validated as per ICH guidelines. Validation parameters studied were linearity and range, recovery study, precision, LOD, LOQ and robustness. Statistical data obtained was found to satisfactorily.