PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells
Christopher A. Reissaus, Kathleen H. Day, Raghavendra G. Mirmira, Kenneth W. Dunn, Fredrick M. Pavalko, Richard N. Day
Topics & Concepts
Förster resonance energy transferBiosensorFluorophoreFluorescence-lifetime imaging microscopyBiophysicsFluorescenceLiving cellFluorescent proteinChemistryNanotechnologyBiological systemBiologyMaterials scienceGreen fluorescent proteinPhysicsBiochemistryOpticsGeneAdvanced Fluorescence Microscopy TechniquesPhotosynthetic Processes and MechanismsAdvanced Electron Microscopy Techniques and Applications