Aflatoxin M1 exposure in a fermented millet-based milk beverage ‘brukina’ and its cancer risk characterization in Greater Accra, Ghana
Nii Korley Kortei, Theophilus Annan, Adjoa Agyemang Boakye, Edward Ken Essuman, Clement Okraku Tettey, Vincent Kyei-Baffour
Abstract
Abstract Brukina is a millet based fermented milk product which is consumed as a beverage in Ghana. It is however prone to aflatoxin M 1 (AFM 1 ) contamination, which is a serious health challenge for low and middle-income countries in subtropical regions. This study aimed at evaluating AFM 1 levels and cancer risks associated with brukina (n = 150) sampled from different locations of the Greater Accra Region of Ghana. AFM 1 were measured with High-Performance Liquid Chromatography (HPLC) connected to a Fluorescence Detector (FLD).Cancer risk assessments were also conducted using models prescribed by the Joint FAO/WHO Expert Committee on Additives (JECFA). Out of the 150 samples analyzed for AFM 1 , 80/150 (53%) tested positive between the range 0.00 ± 0.001–3.14 ± 0.77 µg/kg. Cancer risk assessments of AFM 1 produced outcomes which ranged between 0.64 and 1.88 ng/kg bw/day, 0.31–9.40, 0.0323, and 1.94 × 10 –3 –0.06 for cases/100,000 person/yr for Estimated Daily Intake (EDI), Hazard Index (H.I), Average Potency, and Cancer Risks respectively for all age categories investigated. It was concluded that the consumption of brukina posed adverse health effects on the majority of the age categories in the different locations of Greater Accra Region since the calculated H.Is were greater than one (> 1). Therefore, contamination of brukina with AFM 1 should be considered a high priority in public health and Ghana’s cancer risk management actions.