A simple and rapid mix-and-read assay for sensitive detection of O<sup>6</sup>-methylguanine DNA methyltransferase
Ming‐Hao Liu, Wan‐Tong Yu, Xiaoyun Yang, Yueying Li, Yan Zhang, Chun‐yang Zhang
Abstract
-methylguanine DNA methyltransferase (MGMT) activity based on exonuclease III-assisted signal amplification under completely isothermal conditions (37 °C). This method is very simple and rapid (60 min) with ultrahigh sensitivity and good specificity, and it can detect MGMT activity at the single-cell level. Moreover, this method can be applied for the screening of MGMT inhibitors and the discrimination of MGMT in different cancer cells.
Topics & Concepts
MethyltransferaseDNA methyltransferaseChemistryLoop-mediated isothermal amplificationDNAO-6-methylguanine-DNA methyltransferaseExonucleaseMolecular biologyCombinatorial chemistryBiochemistryBiologyDNA polymeraseMethylationAdvanced biosensing and bioanalysis techniquesInnovative Microfluidic and Catalytic Techniques InnovationCRISPR and Genetic Engineering