Single-Molecule Sampling of Dihydrofolate Reductase Shows Kinetic Pauses and an Endosteric Effect Linked to Catalysis
Nicole Stéphanie Galenkamp, Giovanni Maglia
Abstract
and might be a general feature for complex enzymatic reactions where the binding and release of the products must be tightly controlled.
Topics & Concepts
Dihydrofolate reductaseChemistryCofactorCatalysisEnzyme catalysisEnzymeStereochemistryCatalytic cycleConformational isomerismMoleculeSubstrate (aquarium)ReductaseUmbrella samplingSmall moleculeReaction intermediateCombinatorial chemistryBiochemistryMolecular dynamicsComputational chemistryBiologyOrganic chemistryEcologyNanopore and Nanochannel Transport StudiesAdvanced biosensing and bioanalysis techniquesMicrofluidic and Capillary Electrophoresis Applications