Sensitive identification of neoantigens and cognate TCRs in human solid tumors
Marion Arnaud, Johanna Chiffelle, Raphaël Genolet, Blanca Navarro Rodrigo, Marta A. S. Perez, Florian Huber, Morgane Magnin, Tu Nguyen‐Ngoc, Philippe Guillaume, Petra Baumgaertner, Chloé Chong, Brian J. Stevenson, David Gfeller, Melita Irving, Daniel E. Speiser, Julien Schmidt, Vincent Zoete, Lana E. Kandalaft, Michal Bassani‐Sternberg, Sara Bobisse, George Coukos, Alexandre Harari
Abstract
The identification of patient-specific tumor antigens is complicated by the low frequency of T cells specific for each tumor antigen. Here we describe NeoScreen, a method that enables the sensitive identification of rare tumor (neo)antigens and of cognate T cell receptors (TCRs) expressed by tumor-infiltrating lymphocytes. T cells transduced with tumor antigen-specific TCRs identified by NeoScreen mediate regression of established tumors in patient-derived xenograft mice.