Structural overview of DNA and RNA G-quadruplexes in their interaction with proteins

Abstract

Since the discovery of G-quadruplex (G4) participation in vital cellular processes, the regulation of the interaction of naturally occurring G4s with the relative target proteins has emerged as a promising approach for therapeutic development. Additionally, a synthetic strategy has produced several oligonucleotide aptamers, embodying a G4 module, which exhibit relevant biological activity by binding selectively to a target protein. In this context, the G4-protein structures available in the Protein Data Bank represent a valuable molecular view of the different G4 topologies involved in protein interaction. Interestingly, recent results have shown the co-existence of G4s with other structural domains such as duplexes. Overall, these findings allow a better understanding of the mechanisms that regulate intricate biological functions and suggest new design for innovative medical treatments. • Structural details on the interaction of different G4 topologies with proteins. • Modulation of protein function by the structural versatility of G4s. • Oligonucleotide-protein structures shed light on mixed G4-based architectures.

Topics & Concepts