A Pronucleotide Probe for Live‐Cell Imaging of Protein AMPylation
Pavel Kielkowski, Isabel Y. Buchsbaum, Tobias Becker, Kathrin Bach, Silvia Cappello, Stephan A. Sieber
Abstract
Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.
Topics & Concepts
ChemistryLive cell imagingCellNanotechnologyBiochemistryCombinatorial chemistryBusinessMaterials scienceClick Chemistry and ApplicationsBiotin and Related StudiesAdvanced biosensing and bioanalysis techniques