PBP2b Mutations Improve the Growth of Phage-Resistant <i>Lactococcus cremoris</i> Lacking Polysaccharide Pellicle
H. Guérin, Pascal Quénée, Simon Palussière, Pascal Courtin, Gwenaëlle André, Christine Péchoux, Vlad Costache, Jennifer Mahony, Douwe van Sinderen, Saulius Kulakauskas, Marie‐Pierre Chapot‐Chartier
Abstract
mutants resistant to bacteriophage infection with a restored fitness. This study provides an approach to isolate non-GMO phage-resistant L. cremoris and L. lactis strains, which can be applied to strains with technological functionalities. Also, our results highlight for the first time the link between peptidoglycan and cell wall polysaccharide biosynthesis.
Topics & Concepts
Lactococcus lactisLactococcusMutantBiologyBacteriophagePeptidoglycanMicrobiologyBacteriaCell wallBacterial cell structureStreptococcaceaeBiochemistryGeneGeneticsLactic acidEscherichia coliBacteriophages and microbial interactionsGenomics and Phylogenetic StudiesProbiotics and Fermented Foods