Multi–gene phylogeny and taxonomy of Physisporinus (Polyporales, Basidiomycota)

Abstract

The polypores in Physisporinus are important wood decayers, in which they grow on both angiosperms and gymnosperms from living trees to rotten wood in almost all the forest ecosystems worldwide.The species diversity of the genus has been investigated, but it is still not well known.Phylogenetic and morphological analyses of this genus are carried out based on samples from Asia, Europe, Australia, and North and Central America.This genus is defined by a monomitic hyphal system with simple septa on generative hyphae, hyphoid cystidia present in most species, hyaline, thin-walled, ellipsoid to subglobose basidiospores, and having a white-rotting ecology.Phylogenies are reconstructed by using multiple loci DNA sequences containing ITS, nuc-LSU, nuc-SSU, mt-SSU, TEF1-, and RPB2.The results demonstrate that Physisporinus and Meripilus belong to Meripilaceae of Polyporales.In addition, 12 new species in Physisporinus are illustrated and described, and three unnamed taxa are discussed with a full description.Our phylogeny demonstrates that all taxa of Physisporinus formed five clades nested in a major clade.A key to accepted 37 taxa of Physisporinus is provided. Keywords -Meripilaceaenew taxapolyporephylogenetic analyseswhite rotThe PCR procedures for ITS, nuc-LSU, TEF1, RPB2, nuc-SSU and mt-SSU, and performing of DNA sequences follow Sun et al. (2022) and Wang et al. (2023b).The sequences obtained from specimens involved in this study are uploaded to GenBank.All sequences analysed in this study are listed in Table 1.The final ITS, nuc-LSU, TEF1-, RPB2, nuc-SSU, and mt-SSU datasets are subsequently aligned using MAFFT v.7 under the E-INS-i strategy with no cost for opening gaps and equal cost for transformations (command line: mafft -genafpair -maxiterate 1000) (Katoh & Standley 2013), visualized and manually adjusted in BioEdit (Hall 1999).The different sequence alignments were spliced and transformed formats in Mesquite v.3.2.(Maddison & Maddison 2017).Multiple sequence alignments were trimmed by trimAI v.1.2using thehtmlout-gt 0.8 -st option to deal with gaps, when necessary (Capella-Gutierrez et al. 2009). Phylogenetic analysesIn this study, two datasets were analysed for phylogenies; a two loci DNA fragments dataset (ITS+nuc-LSU) and a six loci DNA fragments dataset (ITS+nuc-LSU+TEF1+RPB2+nuc-SSU+mt-SSU) were used to determine the taxonomic position of the new species in phylogenies, alignments of six loci DNA fragments (ITS+nuc-LSU+TEF1-+RPB2+nuc-SSU+mt-SSU) are concatenated in sequence using Mesquite v.3.2., a total length of 5186 bp, among them 1-676 bp are involved in ITS fragment, 677-2018 bp in nuc-LSU fragment, 2019-2589 bp in TEF1 fragment, 2590-3668 bp in RPB2 fragment, 3669-4694 bp in nuc-SSU fragment and 4695-5186 bp in mt-SSU fragment.The sequence matrices and the obtained trees were uploaded in TreeBase (http://www.treebase.org),under accession ID:

Topics & Concepts