Development of a Novel ATP Bioluminescence Assay Based on Engineered Probiotic <i>Saccharomyces boulardii</i> Expressing Firefly Luciferase
Ji Sun Park, Young-Woo Kim, Hyungdong Kim, Sun‐Ki Kim, Kyeongsoon Park
Abstract
, ATP) in the gut, a colitis mouse model was established using dextran sodium sulfate as a colitogenic compound. Our findings demonstrated that the whole-cell biosensor can detect elevated ATP levels during gut inflammation in mice. Therefore, the simple and powerful method developed herein could be applied for non-invasive IBD diagnosis.
Topics & Concepts
LuciferaseBioluminescenceSaccharomyces boulardiiLuciferinBioluminescence imagingProbioticBiochemistryChemistryMicrobiologyBiologyMolecular biologyGeneBacteriaTransfectionGeneticsbioluminescence and chemiluminescence research