Antimicrobial susceptibility and production of virulence factors by bacteria recovered from bitches with pyometra
Marcos Fábio Gadelha Rocha, Débora D. Q. Paiva, Bruno Rocha Amando, Carliane Melo Alves Melgarejo, Alyne Soares Freitas, Francisco Isaac Fernandes Gomes, Crister José Ocadaque, Cecília Leite Costa, Gláucia M.M. Guedes, Reginaldo Gonçalves de Lima-Neto, Rossana de Aguiar Cordeiro, José Júlio Costa Sidrim, Débora de Souza Collares Maia Castelo‐Branco
Abstract
Pyometra is one of the most common diseases in adult female dogs, characterized by a suppurative bacterial infection of the uterus with accumulation of inflammatory exudate and a variety of local and systemic clinical manifestations. This study aimed to identify the bacteria within the uterine content and vaginal canal of bitches with pyometra and evaluate their antimicrobial susceptibility and production of virulence factors. Uterine and vaginal content were collected with sterile swabs from 30 bitches diagnosed with pyometra. Bacteria were identified and assessed for their antimicrobial susceptibility and production of virulence factors, including biofilms, siderophores, proteases and hemolysins, both in planktonic and biofilm forms. A total of 82 bacterial isolates (35 uterus, 47 vagina), belonging to 21 species, were identified, with Escherichia coli as the most prevalent species (32/82, 39%). As for susceptibility, 39/79 (49.4%) isolates were resistant to one or more drugs, with resistance proportion among Gram-positive bacteria (87.5%) higher (p < .05) than that observed for Gram-negative bacteria (32.7%). Four coagulase-negative Staphylococcus species were resistant to methicillin. Regarding virulence, the isolates had low production of biofilms, siderophores, proteases and hemolysins, suggesting that the occurrence of pyometra might be more associated with host-related factors than bacterial virulence.