Reduced brain oxygen metabolism in patients with multiple sclerosis: Evidence from dual-calibrated functional MRI
Hannah Chandler, Rachael Stickland, Eleonora Patitucci, Michael Germuska, Antonio Maria Chiarelli, Catherine Foster, Shona Bhome-Dhaliwal, T. Lancaster, Neeraj K. Saxena, Sharmila Khot, Valentina Tomassini, Richard G. Wise
Abstract
Cerebral energy deficiency is increasingly recognised as an important feature of multiple sclerosis (MS). Until now, we have lacked non-invasive imaging methods to quantify energy utilisation and mitochondrial function in the human brain. Here, we used novel dual-calibrated functional magnetic resonance imaging (dc-fMRI) to map grey-matter (GM) deoxy-haemoglobin sensitive cerebral blood volume (CBV dHb ), cerebral blood flow (CBF), oxygen extraction fraction (OEF), and cerebral metabolic rate of oxygen consumption (CMRO 2 ) in patients with MS (PwMS) and age/sex matched controls. By integrating a flow-diffusion model of oxygen transport, we evaluated the effective oxygen diffusivity of the capillary network (D C ) and the partial pressure of oxygen at the mitochondria (PmO 2 ). Significant between-group differences were observed as decreased CBF ( p = 0.010), CMRO 2 ( p < 0.001) and D C ( p = 0.002), and increased PmO 2 ( p = 0.043) in patients compared to controls. No significant differences were observed for CBV dHb ( p = 0.389), OEF ( p = 0.358), or GM volume ( p = 0.302). Regional analysis showed widespread reductions in CMRO 2 and D C for PwMS. Our findings may be indicative of reduced oxygen demand or utilisation in the MS brain and mitochondrial dysfunction. Our results suggest changes in brain physiology may precede MRI-detectable GM loss and may contribute to disease progression and neurodegeneration.