Pairing cells of different sizes in a microfluidic device for immunological synapse monitoring
Faruk Azam Shaik, Clara Lewuillon, Aurélie Guillemette, Bahram Ahmadian, Carine Brinster, Bruno Quesnel, Dominique Collard, Yasmine Touil, Loïc Lemonnier, Mehmet C. Tarhan
Abstract
-direction for on-site modulation of effective channel height to capture smaller cells as single cells. Due to immune cells' sensitivity to pressure, the proposed method provides a stable system without any change in flow conditions at the analysis area throughout experiments. Paired live cells have their activities analyzed with calcium imaging at the immunological synapse formed under a controlled environment. The method is demonstrated with primary human T lymphocytes, acute myeloid leukemia (AML) cell lines, and primary AML blasts.
Topics & Concepts
PairingImmunological synapseImmune systemCell biologySensitivity (control systems)CellSynapseCell cultureMyeloidFlow cytometryModulation (music)T cellBiologyChemistryMaterials scienceImmunologyPhysicsNeuroscienceEngineeringSuperconductivityElectronic engineeringT-cell receptorGeneticsAcousticsQuantum mechanicsMicrofluidic and Bio-sensing TechnologiesMicrofluidic and Capillary Electrophoresis ApplicationsImmune Cell Function and Interaction