A ligation-driven CRISPR–Cas biosensing platform for non-nucleic acid target detections
Jiali Zhao, Zhen Tan, Liu Wang, Chunyang Lei, Zhou Nie
Abstract
, ATP and polynucleotide kinase (PNK). In this design, the DNA ligation reaction triggered by these biomolecules generates DNA duplexes, which can activate the nuclease activity of Cas12a to produce amplified fluorescence signals. As a result, this work provides an alternative strategy to expand the applicability of the CRISPR-Cas system into the detection of non-nucleic acid biomolecules.
Topics & Concepts
Nucleic acidCRISPRBiosensorLigationNucleic acid detectionChemistryNanotechnologyDNAComputational biologyCombinatorial chemistryBiologyMaterials scienceBiochemistryMolecular biologyGeneCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesInnovative Microfluidic and Catalytic Techniques Innovation