Plug-and-play adaptive optics for commercial laser scanningfluorescence microscopes based on an adaptive lens
Paolo Pozzi, Martino Quintavalla, Aaron B. Wong, J. Gerard G. Borst, Stefano Bonora, Michel Verhaegen
Abstract
In this Letter, we present a solution for simple implementation of adaptive optics in any existing laser scanning fluorescence microscope. Adaptive optics are implemented by the introduction of a multiactuator adaptive lens between the microscope body and the objective lens. Correction is performed with a sensorless method by optimizing the quality of the images presented on screen by the microscope software. We present the results acquired on both a commercial linear excitation confocal microscope and a custom-made multiphoton excitation microscope.
Topics & Concepts
MicroscopeOpticsLens (geology)4Pi microscopeAdaptive opticsLaser scanningMaterials scienceLaserConfocalFluorescence microscopeOptical microscopeMicroscopyScanning electron microscopeFluorescencePhysicsMultiphoton fluorescence microscopeAdvanced Fluorescence Microscopy TechniquesOptical Coherence Tomography ApplicationsPhotoacoustic and Ultrasonic Imaging