Reliability and Comparison of Sonographic Methods for In Vivo Measurement of Human Biceps Femoris Long-Head Architecture

Abstract

INTRODUCTION: Biceps femoris long-head fascicle length ( Lf ), fascicle angle (FA), and muscle thickness (MT) estimates obtained across 2 d from extended field-of-view (EFOV) sonographic images were compared with those measured from a collage of single ultrasound images (to visualize entire fascicles) as well as a range of geometric equations and extrapolation methods used on single images. Both test validity and intraday reliability were determined. METHODS: Twenty healthy adults (10 men and 10 women) were tested on two occasions (day 1 and day 2), 7 d apart at the same time of day for test-retest measurements. Ultrasound imaging was performed using EFOV and static image acquisition sequences; in the latter, four single images were acquired in-series along the muscle. From these images, Lf was assessed using seven methods: EFOV, collage, manual linear extrapolation, and four different trigonometric equations (termed equations A, B, C, and D), and FA and MT were measured in EFOV, collage, and single images. RESULTS: Lf , FA, and MT measured on days 1 and 2 were not different ( P > 0.05) for any method, reliabilities were very high (intraclass correlation coefficient, 0.91-0.98), and correlations were strong (≥0.84). Significant correlations ( P < 0.05; r = 0.67-0.98) were found between EFOV and the other measurement techniques for Lf , FA, and MT. The collage method had the highest reliability for Lf , and highest rank order and correlation with EFOV. CONCLUSIONS: Although the six different techniques used to estimate Lf provided values similar to EFOV, higher between-subject measurement variability was observed with trigonometric equations, and the collage method described herein provided the most accurate and reliable results and is therefore recommended for biceps femoris long-head architectural analysis when EFOV is not available.

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