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Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression

Duo Shen, Hongyu Zhao, Peng Zeng, Jinyun Song, Yiqiong Yang, Xue‐Feng Gu, Qinghua Ji, Wei Zhao

2020Journal of the Korean Gastric Cancer Association22 citationsDOIOpen Access PDF

Abstract

PURPOSE: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. MATERIALS AND METHODS: ) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. RESULTS: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. CONCLUSIONS: axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.

Topics & Concepts

Downregulation and upregulationCancerCarcinogenesisCell growthCompeting endogenous RNACancer researchCancer cellBiologyCell cultureMedicineGeneGeneticsLong non-coding RNACircular RNAs in diseasesMicroRNA in disease regulationCancer-related molecular mechanisms research