Key Contributions of the Overexpressed<i>Plutella xylostella</i>Sigma Glutathione<i>S</i>-Transferase 1 Gene (<i>PxGSTs1</i>) in the Resistance Evolution to Multiple Insecticides
Jiyuan Liu, Zhen Tian, Ruichi Li, Shujun Ni, Hong Sun, Fei Yin, Zhenyu Li, Yalin Zhang, Yifan Li
Abstract
The overexpression of insect detoxification enzymes is a typical adaptive evolutionary strategy for insects to cope with insecticide pressure. In this study, we identified a glutathione S -transferase (GST) gene, PxGSTs1, that exhibited pronounced expression in the field-resistant population of Plutella xylostella . By using RNAi (RNA interference), the transgenic fly models, and quantitative real-time polymerase chain reaction (RT-qPCR) methods, we confirmed that the augmented expression of PxGSTs1 mediates the resistance of P. xylostella to various types of insecticides, including chlorantraniliprole, novaluron, λ-cyhalothrin, and abamectin. PxGSTs1 was found to bolster insecticide resistance in two ways: direct detoxification and enhancing antioxidative defenses. In addition, our findings demonstrated that pxy-miR-8528a exerts a pivotal influence on forming insecticide resistance in P. xylostella by downregulating PxGSTs1 expression. In summary, we elucidated the multifaceted molecular and biochemical underpinnings of PxGSTs1 -driven insecticide resistance in P. xylostella . Our results provide a new perspective for understanding the insecticide resistance mechanism of P. xylostella .