Ultralow Ru Single Atoms Confined in Cerium Oxide Nanoglues for Highly‐Sensitive and Robust H<sub>2</sub>O<sub>2</sub>‐Related Biocatalytic Diagnosis
Minjia Yuan, Qian Li, Zihe Wu, Huang Zhu, Yang Gao, Mi Zhou, Xianglin Luo, Mao Wang, Chong Cheng
Abstract
Abstract Exploring highly efficient, portable, and robust biocatalysts is a great challenge in colorimetric biosensors. To overcome the challenging states in creating single‐atom biocatalysts, such as insufficient activity and stability, here, this work has engineered a unique CeO 2 support as nanoglue to tightly anchor the Ru single‐atom sites (CeO 2 ‐Ru) with strong electronic coupling for achieving highly sensitive and robust H 2 O 2 ‐related biocatalytic diagnosis. The morphology and chemical/electronic structure analysis demonstrates that the Ru atoms are well‐dispersed on CeO 2 surface to form high‐density active sites. Benefiting from the unique structure, the prepared CeO 2 ‐Ru exhibits outstanding peroxidase (POD) like catalytic activity and selectivity to H 2 O 2 . Steady‐state kinetic study results show that the CeO 2 ‐Ru presents the highest V max and turnover number than the state‐of‐the‐art POD‐like biocatalysts. Consequently, the CeO 2 ‐Ru discloses a high efficiency, good selectivity, and robust stability in the colorimetric detection of L ‐cysteine, glucose, and uric acid. Notably, the limit of detection (LOD) can reach 0.176 × 10 −3 m for the L ‐cysteine, 0.095 × 10 −3 m for the glucose, and 0.088 × 10 −3 m for the uric acid via cascade reaction. This work suggests that the proposed unique CeO 2 nanoglue will offer a new path to create single‐atom noble metal biocatalysts and take a step closer to future biotherapeutic and biocatalytic applications.