High-resolution analysis of the cytosolic Ca <sup>2+</sup> events in β cell collectives in situ
Srdjan Postic, Srdjan Sarikas, Johannes Pfabe, Viljem Pohorec, Lidija Križančić Bombek, Nastja Sluga, Maša Skelin Klemen, Jurij Dolenšek, Dean Korošak, Andraž Stožer, Carmella Evans‐Molina, James D. Johnson, Marjan Slak Rupnik
Abstract
Physiological glucose or ryanodine stimulation of β cell collectives generates a large number of [Ca 2+ ] c events, which can be rapidly assessed with our newly developed automatic image segmentation and [Ca 2+ ] c event identification pipeline. The event durations segregate into three reproducible modes produced by a progressive temporal summation. Using pharmacological tools, we show that activation of ryanodine intracellular Ca 2+ receptors is both sufficient and necessary for glucose-dependent [Ca 2+ ] c oscillations in β cell collectives.