Dual-frequency impedance assays for intracellular components in microalgal cells
Tao Tang, Xun Liu, Yapeng Yuan, Ryota Kiya, Yigang Shen, Tianlong Zhang, Kengo Suzuki, Yo Tanaka, Ming Li, Yoichiroh Hosokawa, Yaxiaer Yalikun
Abstract
, tilt index) successfully characterized the changes in cell shape and intracellular composition distribution. Besides, the electrical opacity showed a stable ratio of the intracellular components to cell volume under the cellular self-regulation. Additionally, simulations were used to support these findings and to elucidate how submicron intracellular components and cell morphology affect impedance signals, providing a basis for future improvements. This work opens up a label-free and high-throughput way to analyze single-cell intracellular components by impedance cytometry.
Topics & Concepts
Electrical impedanceIntracellularDual (grammatical number)Focused Impedance MeasurementChemistryMaterials scienceCell biologyBiologyEngineeringElectrical engineeringArtLiteratureMicrofluidic and Bio-sensing TechnologiesAnalytical Chemistry and Sensors3D Printing in Biomedical Research