The comparison of ZFNs, TALENs, and SpCas9 by GUIDE-seq in HPV-targeted gene therapy
Zifeng Cui, Hui Liu, Hongfeng Zhang, Zhaoyue Huang, Rui Tian, Lifang Li, Weiwen Fan, Yili Chen, Lijie Chen, Sen Zhang, Bhudev C. Das, Konstantin Severinov, Inga I. Hitzeroth, Priya Ranjan Debata, Zhuang Jin, Jiashuo Liu, Zheying Huang, Weiling Xie, Hongxian Xie, Bin Lang, Ji Ma, Haiyan Weng, Xun Tian, Zheng Hu
Abstract
off-target detection pipeline for three generations of artificial nucleases provided useful tools for genome engineering-based gene therapy.
Topics & Concepts
Transcription activator-like effector nucleaseZinc finger nucleaseGenome editingCRISPRCas9BiologyGeneticsComputational biologyGeneCRISPR and Genetic EngineeringPlant Virus Research StudiesMosquito-borne diseases and control