Nanoscale Infrared Spectroscopy and Chemometrics Enable Detection of Intracellular Protein Distribution
A. Catarina V. D. dos Santos, Rosa Heydenreich, Christian Derntl, Astrid R. Mach‐Aigner, Robert L. Mach, Georg Ramer, Bernhard Lendl
Abstract
using the colocation of a fluorescent label (enhanced yellow fluorescence protein, EYFP) with the target enzymes to calibrate the chemometric model. The obtained partial least squares model successfully shows the distribution of these proteins inside the cell and opens the door for further studies on protein secretion mechanisms using PTIR.
Topics & Concepts
ChemistryChemometricsTrichoderma reeseiNanoscopic scalePhotothermal therapyIntracellularResolution (logic)MicroscopyChemical imagingBiological systemPartial least squares regressionFluorescence spectroscopyNanotechnologyAnalytical Chemistry (journal)FluorescenceBiophysicsHyperspectral imagingChromatographyCellulaseOpticsMaterials scienceBiochemistryEnzymeRemote sensingPhysicsComputer scienceMathematicsBiologyGeologyStatisticsArtificial intelligenceSpectroscopy Techniques in Biomedical and Chemical ResearchAdvanced Fluorescence Microscopy TechniquesImage Processing Techniques and Applications