Epithelial and Neutrophil Interactions and Coordinated Response to<i>Shigella</i>in a Human Intestinal Enteroid-Neutrophil Coculture Model
Jose M. Lemme‐Dumit, Michèle Doucet, Nicholas C. Zachos, Marcela F. Pasetti
Abstract
Studies of mucosal immunity and microbial host cell interaction have traditionally relied on animal models and in vitro tissue culture using immortalized cancer cell lines, which yield nonphysiological and often unreliable results. Herein, we report the development and characterization of an ex vivo enteroid-PMN coculture consisting of normal human intestinal epithelium and a mechanistic interrogation of PMN and epithelial cell interaction and function in the context of Shigella infection.
Topics & Concepts
ShigellaMicrobiologyImmunologyNeutrophileNeutrophil extracellular trapsChemistryInflammationBiologyBacteriaSalmonellaGeneticsEscherichia coli research studiesGut microbiota and healthBarrier Structure and Function Studies