Microfluidic 3D Printing Responsive Scaffolds with Biomimetic Enrichment Channels for Bone Regeneration
Xiaocheng Wang, Yunru Yu, Chaoyu Yang, Changmin Shao, Keqing Shi, Luoran Shang, Fangfu Ye, Yuanjin Zhao
Abstract
Figure 5. In vitro osteogenic activity of BP-HF scaffolds. (a) Fluorescent images of rat bone mesenchymal stem cells (rBMSCs) after seeding on the HF and BP-HF scaffolds for 24 h (Green: cytoskeleton, blue: nuclei). Scale bars, 200 m. (b) The cell proliferation of rBMSCs cultured with HF and BP-HF scaffolds for 5 days. (c) Representative images of alkaline phosphatase (ALP) and alizarin red (AR) staining for rBMSCs cultured with HF and BP-HF scaffolds on days 7, 14 and 21. Scale bar, 1 mm. Quantitative analysis of (d) relative ALP activity and (e) calcium deposits mineralized in HF and BP-HF scaffold groups (*p<0.05, **p<0.01, ***p<0.001). BP-HF scaffolds significantly promoted the proliferation and osteogenic differentiation of rBMSCs as compared to the pure HF scaffolds.