α-Amylase and dipeptidyl peptidase-4 (DPP-4) inhibitory effects of <i>Melicope latifolia</i> bark extracts and identification of bioactive constituents using <i>in vitro</i> and <i>in silico</i> approaches
Alexandra Quek, Nur Kartinee Kassim, Pei Cee Lim, Dai Chuan Tan, Muhammad Alif Mohammad Latif, Amin Ismail, Khozirah Shaari, Khalijah Awang
Abstract
CONTEXT: (DC.) T. G. Hartley (Rutaceae) was reported to contain various phytochemicals including coumarins, flavonoids, and acetophenones. OBJECTIVE: bark extracts, fractions, and isolated constituents. MATERIALS AND METHODS: fractions and compounds were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene bleaching assays. RESULTS: : 911.44 μM). DISCUSSION AND CONCLUSIONS: as an alternative source of α-amylase and DPP-4 inhibitors. Further pharmacological studies on the compounds are recommended.
Topics & Concepts
ChemistryDPPHIC50AntioxidantDipeptidyl peptidaseTraditional medicineAmylaseChloroformKaempferolQuercetinBiochemistryEnzymeIn vitroChromatographyMedicinePhytochemical compounds biological activitiesProtein Hydrolysis and Bioactive PeptidesPeptidase Inhibition and Analysis