Highly accurate colorectal cancer prediction model based on Raman spectroscopy using patient serum
Hiroaki Ito, Naoyuki Uragami, Tomokazu Miyazaki, William C. Yang, Kenji Issha, Kai Matsuo, Satoshi Kimura, Yuji Arai, Hiromasa Tokunaga, Saiko Okada, Machiko Kawamura, Noboru Yokoyama, Miki Kushima, Haruhiro Inoue, Takashi Fukagai, Yumi Kamijo
Abstract
BACKGROUND: Colorectal cancer (CRC) is an important disease worldwide, accounting for the second highest number of cancer-related deaths and the third highest number of new cancer cases. The blood test is a simple and minimally invasive diagnostic test. However, there is currently no blood test that can accurately diagnose CRC. AIM: To develop a comprehensive, spontaneous, minimally invasive, label-free, blood-based CRC screening technique based on Raman spectroscopy. METHODS: We used Raman spectra recorded using 184 serum samples obtained from patients undergoing colonoscopies. Patients with malignant tumor histories as well as those with cancers in organs other than the large intestine were excluded. Consequently, the specific diseases of 184 patients were CRC (12), rectal neuroendocrine tumor (2), colorectal adenoma (68), colorectal hyperplastic polyp (18), and others (84). We used the 1064-nm wavelength laser for excitation. The power of the laser was set to 200 mW. RESULTS: values for CRC, adenomas, hyperplastic polyps, and neuroendocrine tumors were 0.9982, 0.9630, 0.9962, and 0.9986, respectively. CONCLUSION: value was constructed. We are currently planning studies to demonstrate the accuracy of this model with a large amount of additional data.