Functional and Biochemical Characterization of the MazEF6 Toxin-Antitoxin System of Mycobacterium tuberculosis
Gopinath Chattopadhyay, Munmun Bhasin, Shahbaz Ahmed, Tannu Priya Gosain, Srivarshini Ganesan, Sayan Das, Chandrani Thakur, Nagasuma Chandra, Ramandeep Singh, Raghavan Varadarajan
Abstract
. To obtain insights into M. tuberculosis MazEF6 type II TA system function, we determined stability, oligomeric states, and binding affinities of cognate partners with each other and with their promoter operator DNA. Using RNA-seq data obtained from M. tuberculosis overexpression strains, we have identified putative MazF6 cleavage sites and targets in its native, cellular context.
Topics & Concepts
AntitoxinBiologyMycobacterium tuberculosisToxinMicrobiologyIn vitroTuberculosisBiochemistryPathologyMedicineTuberculosis Research and EpidemiologyAntibiotic Resistance in BacteriaClostridium difficile and Clostridium perfringens research