Capture of activated dioxygen intermediates at the copper-active site of a lytic polysaccharide monooxygenase
Gabriela C. Schröder, William B. O’Dell, Simon J. Webb, Pratul K. Agarwal, Flora Meilleur
Abstract
Superoxo and hydroperoxo intermediates were cryotrapped at the copper active site of lytic polysaccharide monooxygenase using neutron protein crystallography.
Topics & Concepts
ChemistryProtonationActive siteMonooxygenaseReaction intermediateStereochemistryPhotochemistryMolecular dynamicsHeterolysisCatalysisComputational chemistryOrganic chemistryEnzymeCytochrome P450IonMicrobial bioremediation and biosurfactantsMicrobial Metabolic Engineering and BioproductionMicrobial metabolism and enzyme function