Harmonizing the growing fluorogenic RNA aptamer toolbox for RNA detection and imaging
Xiaocen Lu, Kristen Y. S. Kong, Peter J. Unrau
Abstract
, molar brightness, binding affinity, fluorophore exchange capabilities and other details). General guidelines for choosing fluorescent RNA tools, with an emphasis on single-molecule detection and multi-colour imaging applications are provided. Lastly the importance of global standards for evaluating fluorogenic RNA aptamer systems is discussed.
Topics & Concepts
AptamerRNAComputational biologyToolboxChemistryMolecular biologyBiologyComputer scienceBiochemistryGeneProgramming languageAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanismsRNA Interference and Gene Delivery