A Low-Cost, Thermostable, Cell-Free Protein Synthesis Platform for On-Demand Production of Conjugate Vaccines
Katherine F. Warfel, Asher J. Williams, Derek A. Wong, Sarah E. Sobol, Primit Desai, Jie Li, Yung‐Fu Chang, Matthew P. DeLisa, Ashty S. Karim, Michael C. Jewett
Abstract
(ETEC) O78 O-polysaccharide, a pathogen responsible for diarrheal disease, in immunized mice. We anticipate that our low-cost, thermostable cell-free glycoprotein synthesis system will enable new models of medicine biosynthesis and distribution that bypass cold-chain requirements.
Topics & Concepts
ThermostabilityConjugateBiomanufacturingCell-free protein synthesisChemistryEscherichia coliCold chainBiochemistryFood scienceBiotechnologyBiologyProtein biosynthesisEnzymeMathematicsMathematical analysisGeneBacteriophages and microbial interactionsTransgenic Plants and ApplicationsViral gastroenteritis research and epidemiology