The Fluorescence-Activating and Absorption-Shifting Tag (FAST) Enables Live-Cell Fluorescence Imaging of Methanococcus maripaludis
Eric Hernandez, Kyle C. Costa
Abstract
Methanogenic archaea are important members of anaerobic microbial communities where they catalyze essential reactions in the degradation of organic matter. Developing additional tools for studying the cell biology of these organisms is essential to understanding them at a mechanistic level. Here, we show that FAST, in combination with the fluorogenic ligand HMBR, can be used to monitor protein dynamics in live cells of M. maripaludis. The application of FAST holds promise for future studies focused on the metabolism and physiology of methanogenic archaea.
Topics & Concepts
MethanococcusBiologyFluorescenceEuryarchaeotaBiochemistryMethanomicrobialesBimolecular fluorescence complementationArchaeaFormate dehydrogenaseFluorescence microscopeFluorescence-lifetime imaging microscopyBiophysicsMethanosarcinaEnzymeCofactorYeastPhysicsGeneQuantum mechanicsMicrobial Community Ecology and PhysiologyBacterial Genetics and BiotechnologyBacteriophages and microbial interactions