<p>Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration</p>
Chun-Yi Guan, Tian Shi, Jingli Cao, Xueqin Wang, Xu Ma, Hong‐Fei Xia
Abstract
Purpose: This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients. Materials and Methods: qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function. Results: miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR -α expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-α was observed in patients with GDM and GDM rats. Conclusion: The expression of the placental miR-21-5p , which inhibits cell growth and infiltration by up-regulating PPAR-α , is downregulated in pregnant GDM patients, which in turn may affect the placental function. Keywords: gestational diabetes mellitus, miRNA-21 , insulin resistance, PPAR-α