Structure and catalytic activity of the SAM-utilizing ribozyme SAMURI
Hsuan‐Ai Chen, Takumi Okuda, Ann‐Kathrin Lenz, Carolin P. M. Scheitl, Hermann Schindelin, Claudia Höbartner
Abstract
Abstract Ribozymes that catalyze site-specific RNA modification have recently gained increasing interest for their ability to mimic methyltransferase enzymes and for their application to install molecular tags. Recently, we reported SAMURI as a site-specific alkyltransferase ribozyme using S -adenosylmethionine (SAM) or a stabilized analog to transfer a methyl or propargyl group to N 3 of an adenosine. Here, we report the crystal structures of SAMURI in the postcatalytic state. The structures reveal a three-helix junction with the catalytic core folded into four stacked layers, harboring the cofactor and the modified nucleotide. Detailed structure–activity analyses explain the cofactor scope and the structural basis for site selectivity. A structural comparison of SAMURI with SAM riboswitches sheds light on how the synthetic ribozyme overcomes the strategies of natural riboswitches to avoid self-methylation. Our results suggest that SAM and its analogs may serve as substrates for various RNA-catalyzed reactions, for which the corresponding ribozymes remain to be identified.