Distinct fate, dynamics and niches of renal macrophages of bone marrow or embryonic origins
Fengming Liu, Shen Dai, Dechun Feng, Zhongnan Qin, Xiao Peng, Siva S. V. P. Sakamuri, Mi Ren, Li Huang, Min Cheng, Kabir E. Mohammad, Ping Qu, Yong Chen, Chunling Zhao, Faliang Zhu, Shujian Liang, Bertal H. Aktas, Xiaofeng Yang, Hong Wang, Prasad V. G. Katakam, David W. Busija, Tracy Fischer, Prasun K. Datta, Jay Rappaport, Bin Gao, Xuebin Qin
Abstract
Renal macrophages (RMs) participate in tissue homeostasis, inflammation and repair. RMs consist of embryo-derived (EMRMs) and bone marrow-derived RMs (BMRMs), but the fate, dynamics, replenishment, functions and metabolic states of these two RM populations remain unclear. Here we investigate and characterize RMs at different ages by conditionally labeling and ablating RMs populations in several transgenic lines. We find that RMs expand and mature in parallel with renal growth after birth, and are mainly derived from fetal liver monocytes before birth, but self-maintain through adulthood with contribution from peripheral monocytes. Moreover, after the RMs niche is emptied, peripheral monocytes rapidly differentiate into BMRMs, with the CX3CR1/CX3CL1 signaling axis being essential for the maintenance and regeneration of both EMRMs and BMRMs. Lastly, we show that EMRMs have a higher capacity for scavenging immune complex, and are more sensitive to immune challenge than BMRMs, with this difference associated with their distinct glycolytic capacities.