Spatiotemporal Distribution of the Environmental Microbiota in Food Processing Plants as Impacted by Cleaning and Sanitizing Procedures: the Case of Slaughterhouses and Gaseous Ozone
Cristian Botta, Ilario Ferrocino, Alessandro Pessione, Luca Cocolin, Kalliopi Rantsiou
Abstract
survey demonstrates that RNA-based sequencing of 16S rRNA amplicons is a reliable approach to qualitatively probe, at high taxonomic resolution, the changes triggered by new and existing cleaning/sanitizing strategies in the environmental microbiota in human-built environments. This approach could soon represent a fast tool to clearly define which routine sanitizing interventions are more suitable for a specific food processing environment, thus limiting the costs of special cleaning interventions and potential product loss.
Topics & Concepts
LimitingAmplicon sequencingAmpliconFood processingBiologyMicrobiomeEnvironmental science16S ribosomal RNAFood scienceEngineeringBioinformaticsBacteriaPolymerase chain reactionMechanical engineeringGeneGeneticsBiochemistryListeria monocytogenes in Food SafetyFood Safety and HygieneAdvanced Chemical Sensor Technologies