Surgical Treatment for Colorectal Cancer Partially Restores Gut Microbiome and Metabolome Traits
Hirotsugu Shiroma, Satoshi Shiba, Pande Putu Erawijantari, Hiroyuki Takamaru, Masayoshi Yamada, Taku Sakamoto, Yukihide Kanemitsu, Sayaka Mizutani, Tomoyoshi Soga, Yutaka Saito, Tatsuhiro Shibata, Shinji Fukuda, Shinichi Yachida, Takuji Yamada
Abstract
The gut microbiome and metabolites are associated with CRC progression and carcinogenesis. Postoperative CRC patients are reported to be at an increased CRC risk; however, how gut microbiome and metabolites are related to CRC risk in postoperative patients remains only partially understood. In this study, we investigated the influence of surgical CRC treatment on the gut microbiome and metabolites. We found that the CRC-associated species Fusobacterium nucleatum was decreased postsurgery, whereas carcinogenesis-associated DCA and its producing species and genes were increased postsurgery. We developed methods to estimate postoperative CRC risk based on the gut microbiome and metabolomic compositions. We applied methods to compare the estimated CRC risk between two groups according to the presence of large adenoma or tumors after 5 years postsurgery. To our knowledge, this study is the first report on differences between pre- and postsurgery using metagenomics and metabolomics data analysis. Our methods might be used for CRC risk assessment in postoperative patients.